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Extraction of Chromosomal DNA and Plasmid from Yersinia pestis

  • Yazhou Zhou
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Yersinia pestis DNA is needed for genome sequencing, molecular cloning, protein expression, PCR detection, and other experiments. We can use the SDS-proteinase K and Tris-phenol methods to isolate Y. pestis chromosomal DNA and the SDS-based method to purify Y. pestis plasmid DNA. An alternative is the use of commercial kits, which are quick and simple to use. After chromosomal and plasmid DNA extraction, the quality and quantity of extracted DNA should be evaluated.

Key words

Yersinia pestis Chromosomal DNA Plasmid Extraction Quality and quantity determination 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yazhou Zhou
    • 1
  1. 1.Beijing Institute of Microbiology and EpidemiologyBeijingChina

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