Immunological Methods for Detection and Identification of Yersinia pestis

  • Yong Zhao
Part of the Springer Protocols Handbooks book series (SPH)


Yersinia pestis can be identified in the laboratory by conventional bacteriologic test; however, the process is lengthy (at least 2 days). Alternatively, immunological techniques can rapidly detect and identify the organism. This chapter describes methods for Y. pestis identification by immunological methods, including reverse indirect hemagglutination assay (RIHA), enzyme-linked immunosorbent assay (ELISA), immunochromatographic assay (ICA), and immunological biosensor technique.

Key words

Yersinia pestis Immunological techniques Biosensor 


  1. 1.
    Prentice MB, Rahalison L (2007) Plague. Lancet 369(9568):1196–1207CrossRefPubMedGoogle Scholar
  2. 2.
    Perry RD, Fetherston JD (1997) Yersinia pestis – etiologic agent of plague. Clin Microbiol Rev 10(1):35–66PubMedPubMedCentralGoogle Scholar
  3. 3.
    Yan Z, Zhou L, Zhao Y, Wang J, Huang L, Hu K, Liu H, Wang H, Guo Z, Song Y (2006) Rapid quantitative detection of Yersinia pestis by lateral-flow immunoassay and up-converting phosphor technology-based biosensor. Sensors Actuators B Chem 119(2):656–663CrossRefGoogle Scholar
  4. 4.
    Zhang P, Liu X, Wang C, Zhao Y, Hua F, Li C, Yang R, Zhou L (2014) Evaluation of up-converting phosphor technology-based lateral flow strips for rapid detection of Bacillus anthracis Spore, Brucella spp., and Yersinia pestis. PLoS One 9(8):e105305CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Cao LK, Anderson GP, Ligler FS, Ezzell J (1995) Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor. J Clin Microbiol 33(2):336–341PubMedPubMedCentralGoogle Scholar
  6. 6.
    Wei H, Zhao Y, Bi Y, Liu H, Guo Z, Song Y, Zhai J, Huang H, Yang R (2007) Direct detection of Yersinia pestis from the infected animal specimens by a fiber optic biosensor. Sensors Actuators B Chem 123(1):204–210CrossRefGoogle Scholar
  7. 7.
    Yang Y, Wang M, Zhao X, Zhao Z, Zhang A, Wei R, Wei B, Wang Z (2014) Analysis on the results of etiology and serology of plague in Qinghai province from 2001 to 2010. Zhonghua yu fang yi xue za zhi [Chinese J Prev Med] 48(2):124–127Google Scholar
  8. 8.
    Wang ZY, Dai RX, Wei BQ, Qi ZZ, Yang XY, Xin YQ, Zhang SH (2006) Plague diagnosis: reverse indirect hemagglutination assay versus bacteria culture. Chin J Endemiol 33(13):651–664Google Scholar
  9. 9.
    Lindsey CY, Powell BS, Bolt CR, Brown JE, Adamovicz JJ (2012) Validation of quantitative ELISAS for measuring anti-Yersinia pestis F1 and V antibody concentrations in nonhuman primate sera. J Immunoass Immunochem 33(1):91–113CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yong Zhao
    • 1
  1. 1.Beijing Institute of Microbiology and EpidemiologyBeijingChina

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