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PCR for Detection and Identification of Yersinia pestis

  • Yong Zhao
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Molecular methods aimed at Yersinia pestis detection and identification mainly rely on the polymerase chain reaction (PCR) technique, including conventional PCR and real-time PCR. PCR-based assays are more sensitive and rapid than traditional methods, as the isolation and growth of Y. pestis from specimens are laborious and time-consuming. Y. pestis target gene sequences can also be quantified using real-time PCR. In this chapter, methods to extract Y. pestis genomic DNA from simulated soil samples are described. PCR and real-time PCR methods for sensitive and quantitative detection of Y. pestis are also discussed.

Key words

Yersinia pestis Molecular diagnosis PCR assay 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yong Zhao
    • 1
  1. 1.Beijing Institute of Microbiology and EpidemiologyBeijingChina

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