PCR for Detection and Identification of Yersinia pestis
Molecular methods aimed at Yersinia pestis detection and identification mainly rely on the polymerase chain reaction (PCR) technique, including conventional PCR and real-time PCR. PCR-based assays are more sensitive and rapid than traditional methods, as the isolation and growth of Y. pestis from specimens are laborious and time-consuming. Y. pestis target gene sequences can also be quantified using real-time PCR. In this chapter, methods to extract Y. pestis genomic DNA from simulated soil samples are described. PCR and real-time PCR methods for sensitive and quantitative detection of Y. pestis are also discussed.
Key wordsYersinia pestis Molecular diagnosis PCR assay
- 8.Qi Z, Wu Y, Li Y, Li C, Yang X, Zhang Q, Xin Y, Jin Y, Wei R, Cui Y (2015) 3a-negative Yersinia pestis, China. Infec Dis Transl Med 1(2):61–62Google Scholar