Screening of Regulator Responses to Yersinia pestis Infection with a Concatenated Tandem Array of Transcription Factor Response Element (catTFRE) Pulldown
The main methods used to screen transcription factors are RNA-seq, promoter analysis, and chromatin immunoprecipitation (ChIP). Previous studies have constructed transcription factor action models, but some crucial questions remain unresolved. The information obtained from mRNA profiling is inadequate for predicting the activities of transcription factors at the proteomic scale, and ChIP-seq produces only limited transcription factor data at a time because of the constraints on experimental throughput. How can transcription factors be effectively screened and identified at the proteome level? A concatenated tandem array of transcription factor response elements (catTFREs) pulldown technique can be used to identify and assess the binding activities of transcription factors to DNA at the proteomic scale. In this chapter, the determination of the transcription factor responses to Yersinia pestis infection with a catTFRE pulldown technique is described.
Key wordsTranscription factor Pathogenesis Yersinia pestis catTFRE pulldown
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