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Genetic Regulation Analysis of Yersinia pestis

  • Yiquan Zhang
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Gene regulation at the transcriptional level plays important roles in bacterial physiology, survival against stresses, and pathogenesis. In the previous studies, I employed a set of methods, including a microarray assay, an electrophoretic mobility shift assay, a DNase I footprinting assay, a primer extension assay, a LacZ fusion assay, and a real-time reverse transcription–polymerase chain reaction, to elucidate the molecular mechanisms of gene expression at the transcriptional level. These methods allow us to easily identify a transcriptional factor’s regulon and the transcriptional regulation mechanisms of target genes by a transcriptional factor. Here I describe detailed protocols of these methods.

Key words

Gene expression Regulation Transcriptional factor 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yiquan Zhang
    • 1
  1. 1.School of MedicineJiangsu UniversityZhenjiangChina

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