Genetic Regulation Analysis of Yersinia pestis
Gene regulation at the transcriptional level plays important roles in bacterial physiology, survival against stresses, and pathogenesis. In the previous studies, I employed a set of methods, including a microarray assay, an electrophoretic mobility shift assay, a DNase I footprinting assay, a primer extension assay, a LacZ fusion assay, and a real-time reverse transcription–polymerase chain reaction, to elucidate the molecular mechanisms of gene expression at the transcriptional level. These methods allow us to easily identify a transcriptional factor’s regulon and the transcriptional regulation mechanisms of target genes by a transcriptional factor. Here I describe detailed protocols of these methods.
Key wordsGene expression Regulation Transcriptional factor
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