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Plasmid Curing in Yersinia pestis

  • Yazhou Zhou
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Typically, Yersinia pestis contains three plasmids: pCD1, pMT1, and pPCP1. Although there have been many studies of the functions of these plasmids, little is known about how these plasmids act coordinately to promote the virulence of Y. pestis. Therefore, the construction of plasmid-less mutant strains is critical for further investigations of the relationships among these plasmids. In this chapter, I describe a method of curing plasmids from Y. pestis using plasmid incompatibility.

Key words

Curing Incompatibility Plasmid Yersinia pestis 

References

  1. 1.
    Perry RD, Fetherston JD (1997) Yersinia pestis – etiologic agent of plague. Clin Microbiol Rev 10(1):35–66PubMedPubMedCentralGoogle Scholar
  2. 2.
    Prentice MB, Rahalison L (2007) Plague. Lancet 369(9568):1196–1207CrossRefPubMedGoogle Scholar
  3. 3.
    Du Y, Rosqvist R, Forsberg A (2002) Role of fraction 1 antigen of Yersinia pestis in inhibition of phagocytosis. Infect Immun 70(3):1453–1460CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Hinnebusch BJ, Fischer ER, Schwan TG (1998) Evaluation of the role of the Yersinia pestis plasminogen activator and other plasmid-encoded factors in temperature-dependent blockage of the flea. J Infect Dis 178(5):1406–1415CrossRefPubMedGoogle Scholar
  5. 5.
    Zhou D, Tong Z, Song Y, Han Y, Pei D, Pang X, Zhai J, Li M, Cui B, Qi Z et al (2004) Genetics of metabolic variations between Yersinia pestis biovars and the proposal of a new biovar, microtus. J Bacteriol 186(15):5147–5152CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Hutchens M, Luker GD (2007) Applications of bioluminescence imaging to the study of infectious diseases. Cell Microbiol 9(10):2315–2322CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Ni B, Du Z, Guo Z, Zhang Y, Yang R (2008) Curing of four different plasmids in Yersinia pestis using plasmid incompatibility. Lett Appl Microbiol 47(4):235–240CrossRefPubMedGoogle Scholar
  8. 8.
    Uraji M, Suzuki K, Yoshida K (2001) A new method for construction of Ti plasmid-less strains in Agrobacterium tumefaciens. Nucleic Acids Res Suppl 1:173–174CrossRefGoogle Scholar
  9. 9.
    Uraji M, Suzuki K, Yoshida K (2002) A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens. Genes Genet Syst 77(1):1–9CrossRefPubMedGoogle Scholar
  10. 10.
    Qian G, Wang Y, Qian D, Fan J, Hu B, Liu F (2012) Selection of available suicide vectors for gene mutagenesis using chiA (a chitinase encoding gene) as a new reporter and primary functional analysis of chiA in Lysobacter enzymogenes strain OH11. World J Microbiol Biotechnol 28(2):549–557CrossRefPubMedGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Yazhou Zhou
    • 1
  1. 1.Beijing Institute of Microbiology and EpidemiologyBeijingChina

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