Plasmid Curing in Yersinia pestis
Typically, Yersinia pestis contains three plasmids: pCD1, pMT1, and pPCP1. Although there have been many studies of the functions of these plasmids, little is known about how these plasmids act coordinately to promote the virulence of Y. pestis. Therefore, the construction of plasmid-less mutant strains is critical for further investigations of the relationships among these plasmids. In this chapter, I describe a method of curing plasmids from Y. pestis using plasmid incompatibility.
Key wordsCuring Incompatibility Plasmid Yersinia pestis
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