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Isolation and Identification of Yersinia pestis

  • Zhizhen Qi
  • Qingwen Zhang
  • Yong Jin
  • Youquan Xin
  • Haihong Zhao
  • Xiaoyan Yang
  • Xiaoqing Xu
  • Xuefei Zhang
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The isolation and identification of Yersinia pestis are critical for plague surveillance and diagnosis. This pathogen can be isolated from animals and patients using different kinds of specimens and different cultivation strategies. Yersinia pestis can be identified by its biochemical features, by its susceptibility to bacteriophage lysis, by animal experiments, or via polymerase chain reaction. In this chapter, the techniques used to isolate suspected Y. pestis, as well as the methods to identify this pathogen, are described.

Key words

Bacteriophage lysis Biochemical features Identification Isolation PCR Yersinia pestis 

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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Zhizhen Qi
    • 1
  • Qingwen Zhang
    • 1
  • Yong Jin
    • 1
  • Youquan Xin
    • 1
  • Haihong Zhao
    • 1
  • Xiaoyan Yang
    • 1
  • Xiaoqing Xu
    • 1
  • Xuefei Zhang
    • 1
  1. 1.Key Laboratory for Plague Prevention and Control of Qinghai ProvinceQinghai Institute for Endemic Diseases Prevention and ControlXiningChina

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