Bivalent Diabodies

  • Roland E. KontermannEmail author
Part of the Springer Protocols Handbooks book series (SPH)


Single-chain Fv fragments exhibiting monovalent binding can be converted into bivalent molecules by reducing the linker connecting the two variable domains to approximately five residues resulting in homodimerization of two polypeptide chains. This protocol describes the cloning steps to convert an scFv into a diabody molecule and provides some information for production and analysis.


Antibody Fragment SacI Site Calf Intestine Alkaline Phosphatase Antibody Sequence Restriction Site Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Institut für Zellbiologie und ImmunologieUniversität StuttgartStuttgartGermany

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