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In this chapter, we describe the generation of immunoliposomes by means of two different methods applying different scFv formats. The cloning strategies of these scFv variants possessing an additional cysteine residue for site-directted coupling are also presented in this chapter. Furthermore, a procedure for preparation and analysis of drug-loaded immunoliposomes is presented.
KeywordsLipid Film Coupling Buffer PEGylated Liposome Sepharose CL4B Column scFv Gene
- Iden DL, Allen TM (2001) In vitro and in vivo comparison of immunoliposomes made by conventional coupling technique with those made by a new post-insertion approach. Biochim Biophys Acta 1531:207–216Google Scholar