• Sylvia K. E. MesserschmidtEmail author
  • Julia Beuttler
  • Miriam Rothdiener
Part of the Springer Protocols Handbooks book series (SPH)


In this chapter, we describe the generation of immunoliposomes by means of two different methods applying different scFv formats. The cloning strategies of these scFv variants possessing an additional cysteine residue for site-directted coupling are also presented in this chapter. Furthermore, a procedure for preparation and analysis of drug-loaded immunoliposomes is presented.


Lipid Film Coupling Buffer PEGylated Liposome Sepharose CL4B Column scFv Gene 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Sylvia K. E. Messerschmidt
    • 1
    Email author
  • Julia Beuttler
    • 1
  • Miriam Rothdiener
    • 1
  1. 1.Institute of Cell Biology and ImmunologyUniversity of StuttgartStuttgartGermany

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