Production of Antibodies and Antibody Fragments in Escherichia coli
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Antibodies are an important and growing family of approved therapeutic drugs. Antibody fragments of various types as well as more traditional monoclonal antibodies can be produced using E. coli as the production host. This chapter provides protocols for producing antibody fragments and full-length antibodies in E. coli.
KeywordsHeavy Chain Shake Flask Luria Bertani Antibody Fragment Nonreduced Sample
We would like to thank Art Huang, James Giulianotti, and Bryant McLaughlin for Western blots.
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