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Expressing Intrabodies in Mammalian Cells

  • Alessio Cardinale
  • Silvia BioccaEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The intrabody approach is a gene-based technology developed to neutralize or modify the function of intracellular and extracellular target antigens. Since intrabodies can potentially target all different multiple isoforms of a protein, they can act as therapeutic molecules for the treatment of viral pathologies, cancer, and misfolding diseases. This chapter provides protocols for application of intrabodies in mammalian cells. We describe all the steps to express intrabodies in different subcellular compartments and to analyze the biochemical properties of scFv fragments and their intracellular distribution. We focus on protocols to study the antigen–intrabody complex in the secretory compartment and to analyze the effects of intrabody expression on membrane target protein.

Keywords

PC12 Cell Prion Protein Tissue Culture Dish Wash Cell Cell Growth Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  1. 1.Department of NeuroscienceUniversity of Rome “Tor Vergata”RomaItaly
  2. 2.IRCCSSan RaffaeleRomaItaly

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