Structural Characterization of Antibodies by Mass Spectrometry

  • Marie-Claire Janin-Bussat
  • Jean-Marc Strub
  • Elsa Wagner-Rousset
  • Olivier Colas
  • Christine Klinguer-Hamour
  • Nathalie Corvaia
  • Alain van Dorsselaer
  • Alain BeckEmail author
Part of the Springer Protocols Handbooks book series (SPH)

Among all analytical methods used to characterize monoclonal antibodies (mAbs), mass spectrometry plays an increasingly important role for both global and fine structural characterization of therapeutic candidates. In this chapter we discuss and provide detailed protocols for intact antibody and light and heavy chain primary structure characterisation by LC-ESI-TOF, glycosylation and production-system fingerprinting, structural isotyping and disulfide connection determination (humanised IgG1 vs IgG4) and finally non-stable “hot spots” mapping by Mass Spectrometry (de-amidation by MALDI-TOF and LC-IT MS/MS).


Heavy Chain Pyroglutamic Acid Intact Antibody Nominal Molecular Weight Deamidation Site 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Marie-Claire Janin-Bussat
    • 1
  • Jean-Marc Strub
    • 2
  • Elsa Wagner-Rousset
    • 1
  • Olivier Colas
    • 1
  • Christine Klinguer-Hamour
    • 1
  • Nathalie Corvaia
    • 1
  • Alain van Dorsselaer
    • 2
  • Alain Beck
    • 1
    Email author
  1. 1.Centre d’Immunologie Pierre Fabre (CIPF)Saint-Julien-en-GenevoisFrance
  2. 2.Laboratoire de Spectrométrie de Masse Biologique (LSMBO)Université de StrasbourgStrasbourgFrance

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