Protein A/G Chromatography
Part of the
Springer Protocols Handbooks
book series (SPH)
The following chapter describes the purification of whole IgG by Protein A affinity chromatography from crude protein mixtures such as serum, ascites fluids or cell culture supernatant. This reliable method is based on the ability of Protein A to bind with high affinity to the Fc region of immunoblobulins.
KeywordsElution Buffer Cell Culture Supernatant Column Volume Ammonium Sulphate Precipitation Cyanogen Bromide
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