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Protein A/G Chromatography

  • Kirstin A. ZettlitzEmail author
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

The following chapter describes the purification of whole IgG by Protein A affinity chromatography from crude protein mixtures such as serum, ascites fluids or cell culture supernatant. This reliable method is based on the ability of Protein A to bind with high affinity to the Fc region of immunoblobulins.

Keywords

Elution Buffer Cell Culture Supernatant Column Volume Ammonium Sulphate Precipitation Cyanogen Bromide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Deisenhofer J (1981) Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8-A resolution. Biochemistry 20(9):2361–2370PubMedCrossRefGoogle Scholar
  2. Johansson SG, Inganas M (1978) Interaction of polyclonal human IgE with protein-A from Staphylococcus aureus. Immunol Rev 41:248–260PubMedCrossRefGoogle Scholar
  3. Langone JJ (1982) Applications of immobilized protein A in immunochemical techniques. J Immunol Methods 55(3):277–296PubMedCrossRefGoogle Scholar
  4. Lindmark R, Thoren-Tolling K et al (1983) Binding of immunoglobulins to protein A and immunoglobulin levels in mammalian sera. J Immunol Methods 62(1):1–13PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Institute of Cell Biology and ImmunologyUniversity of StuttgartStuttgartGermany

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