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Expression of IgA Molecules in Mammalian Cells

  • Thomas Beyer
  • Stefan Lohse
  • Michael Dechant
  • Thomas ValeriusEmail author
Protocol
  • 3.3k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Although natural IgA antibodies constitute an important immune defense mechanism at serosal surfaces, their immunotherapeutic potential has not been thoroughly explored. Among the limitations hampering the development of therapeutic IgA antibodies is the lack of well established production and purification technologies. Here, we describe protocols to produce and purify recombinant chimeric IgA antibodies irrespective of their antigen specificity. Resulting antibodies were monomeric, fully functional and stable. The obtained production rates and purities will allow further testing e.g. in animal models. Intentionally, we aimed to employ methods similar to those used for IgG production and purification to allow further optimization and up-scaling according to established methods.

Keywords

Glutamine Synthetase Chinese Hamster Ovary Size Exclusion Chromatography Roller Bottle Flat Bottom Tissue Culture Plate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Thomas Beyer
    • 1
  • Stefan Lohse
    • 2
  • Michael Dechant
    • 1
  • Thomas Valerius
    • 3
    Email author
  1. 1.Division of Nephrology and HypertensionChristian-Albrechts-UniversityKielGermany
  2. 2.Division of Stem Cell Transplantation and ImmunotherapyChristian-Albrechts-UniversityKielGermany
  3. 3.Division of Stem Cell Transplantation and ImmunotherapyChristian-Albrechts-UniversityKielGermany

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