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cenM-FISH Approaches

  • Nadezda KosyakovaEmail author
  • Anja Weise
  • Kristin Mrasek
  • Hasmik Mkrtchyan
  • Vivien Klaschka
  • Elisabeth Ewers
  • Daniela Reich
  • Nadine Rüdiger
  • Thomas Liehr
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Multicolor FISH using whole-chromosome painting and FISH banding with partial chromosome painting probes are, for technical reasons, not suited to the characterization of small centromeric and pericentromeric rearrangements. Thus, probe sets for characterizing the different human centromeres, and others that enable studies of the near-centromere regions of human chromosomes, were established. Such centromere-specific multicolor (cenM-) and subcentromere-specific (subcenM-) FISH approaches are presented in this chapter. The preparation and use of these probe sets is reported.

Keywords

Acrocentric Chromosome Chromosomal Breakpoint Chromosomal Origin Human Centromere Small Supernumerary Marker Chromosome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  • Nadezda Kosyakova
      Email author
    • Anja Weise
      • Kristin Mrasek
        • Hasmik Mkrtchyan
          • 1
        • Vivien Klaschka
          • Elisabeth Ewers
            • Daniela Reich
              • Nadine Rüdiger
                • Thomas Liehr
                  1. 1.Department of Genetic and Laboratory of CytogeneticsState UniversityArmeniaJerewan

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