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Molecular Cytogenetics: The Standard FISH and PRINS Procedure

  • Thomas LiehrEmail author
  • Franck Pellestor
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

In this chapter, the two basic principal techniques of molecular cytogenetics are outlined, i.e., fluorescence in situ hybridization (FISH) and primed in situ hybridization (PRINS). Basic protocols are provided, which can be varied and adapted according to specific application(s).

Keywords

Down Syndrome Gentle Agitation Nick Translation Rubber Cement Antifade Solution 
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Notes

Acknowledgments

Supported in parts by the Dr. Robert Pfleger-Stiftung, the INTAS (AISbl 03-51-4060), Stiftung Leukämie and Stefan-Morsch-Stiftung.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Service de Médecine et Biologie de la Reproduction, CHRU Montpellier, Hopital Arnaud de VilleneuveINSERM U847Montpellier Cedex 5France

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