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Characterization of Archived Formalin-Fixed/Paraffin-Embedded or Cryofixed Tissue, Including Nucleus Extraction

  • Thomas LiehrEmail author
  • Marina Manvelyan
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Interphase cytogenetics using archival tissue samples is a straightforward approach that can be used to obtain cytogenetic information from nuclei of solid tissue samples. It is the only tool available for investigating specific numerical chromosomal aberrations, chromosomal translocations, amplification of oncogenes, or the deletion of tumor suppressor genes in archival tissue samples at the single-cell level, as metaphase spreads obviously cannot be obtained. Here a collection of protocols for archival sectioned and mounted materials as well as extracted nuclei (from formalin-fixed/paraffin-embedded or cryofixed tissues) are presented.

Keywords

Nuclear Extraction Abort Fetus Sodium Thiocyanate Small Supernumerary Marker Chromosome Interphase Cytogenetic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Supported in part by the DFG (436 ARM 17/11/06, LI 820/15–1).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Department of Genetic and Laboratory of CytogeneticsState UniversityJerewan

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