Preparation of Tissue and Sections
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The following portion of this book represents a coherent system of quantitative analysis for histochemical purposes. It derives from the original system of Linderstrom-Lang and Holter (1), but differs in several significant details. In the original methodology of the Carlsberg Laboratory, serial frozen sections were cut from a uniform tissue block. Alternate sections were either analyzed, or fixed and stained for histological control. This procedure has been further developed and exploited with great success by David Glick, and is fully described in book form (2).
KeywordsPyridine Nucleotide Dewar Flask Serial Freeze Section Vapor Tension Histological Control
- 1.Linderstrom-Lang, K., Holter, H., and Ohlsen, A. S. (1935) Studies on enzymatic histochemistry XIII. The distribution of enzymes in the stomach of pigs. Compt.-Rend. Lab. Carlsberg, Serie Chim. 20, 66–125.Google Scholar
- 2.Glick, D. (1961,1963) Quantitive Chemical Techniques of Histo-and Cytochemistry, vols. 1 and 2 (Wiley [Interscience]), New York.Google Scholar
- 7.Wenger, B. (1955) Personal communication.Google Scholar
- 10.Hintz, C. S., Lowry, C. V., Kaiser, K. K., McKee, D., and Lowry, O. H. Enzyme levels in individual rat muscle fibers. Am. J. Physiol. 239, C58–C65.Google Scholar