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Immunoelectrophoretic Techniques

2-D Immunoelectrophoresis and Rocket Immunoelectrophoresis
  • John M. Walker
Protocol
  • 687 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

This experiment introduces the student to two commonly used immunoelectrophoretic techniques; rocket immunoelectrophoresis, also known as electroimmunoassay, and 2-D immunoelectrophoresis.

Keywords

Glass Plate Electrophoresis Apparatus Cooling Plate Barbitone Buffer Glass Block 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Laurell, C-B. (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15, 45–52.PubMedCrossRefGoogle Scholar
  2. 2.
    Minchin Clarke, H.G. and Freeman, T. (1968) Quantitative immunoelectrophoresis of human serum proteins. Clin. Sci. 35, 403–413.Google Scholar
  3. 3.
    Dako Information Sheet No. 4. Provided by DAKO-immunoglobulins a/s, Copenhagen, Denmark (or local supplier).Google Scholar

Further Reading

  1. A Manual of Quantitative Immunoelectrophoresis-Methods and Applications (1973) Scand. J. Immunol 2,(Suppl. 1) (Axelsen, N.H., Kroll, J., and Week, B., eds.) Universitatsforgleget, Oslo.Google Scholar
  2. Handbook of Immunoprecipitation-in-Gel Techniques (1983) (Axelsen, N.H., Ed.) Blackwell Scientific Publications.Google Scholar

Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • John M. Walker
    • 1
  1. 1.Division of Biological and Environmental StudiesThe Hatfield PolytechnicUK

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