Immunoelectrophoretic Techniques

2-D Immunoelectrophoresis and Rocket Immunoelectrophoresis
  • John M. Walker
Part of the Springer Protocols Handbooks book series (SPH)


This experiment introduces the student to two commonly used immunoelectrophoretic techniques; rocket immunoelectrophoresis, also known as electroimmunoassay, and 2-D immunoelectrophoresis.


Glass Plate Electrophoresis Apparatus Cooling Plate Barbitone Buffer Glass Block 
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  1. 1.
    Laurell, C-B. (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal. Biochem. 15, 45–52.PubMedCrossRefGoogle Scholar
  2. 2.
    Minchin Clarke, H.G. and Freeman, T. (1968) Quantitative immunoelectrophoresis of human serum proteins. Clin. Sci. 35, 403–413.Google Scholar
  3. 3.
    Dako Information Sheet No. 4. Provided by DAKO-immunoglobulins a/s, Copenhagen, Denmark (or local supplier).Google Scholar

Further Reading

  1. A Manual of Quantitative Immunoelectrophoresis-Methods and Applications (1973) Scand. J. Immunol 2,(Suppl. 1) (Axelsen, N.H., Kroll, J., and Week, B., eds.) Universitatsforgleget, Oslo.Google Scholar
  2. Handbook of Immunoprecipitation-in-Gel Techniques (1983) (Axelsen, N.H., Ed.) Blackwell Scientific Publications.Google Scholar

Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • John M. Walker
    • 1
  1. 1.Division of Biological and Environmental StudiesThe Hatfield PolytechnicUK

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