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Isolation of Plasmid DNA

The Cleared Lysate and CsCl Gradient Technique
  • Stephen A. Boffey
Protocol
  • 754 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Plasmids first hit the scientific headlines when it was discovered that they are the means by which bacteria are able to transfer properties, such as resistance to an antibiotic, from one cell to another. It is now known that plasmids are circular, double-stranded molecules of non-chromosomal DNA that contain their own origin of replication, and can therefore replicate independently within the bacterial cell. They are considerably smaller than the chromosomal DNA, but can contain genes, not only for antibiotic resistance, but also for antibiotic synthesis, toxin production, nitrogen fixation, production of degradative enzymes, and conjugation. The plasmids can be transferred from one cell to another, and therefore act as carriers or “vectors” of the extrachromosomal genes they contain.

Keywords

Dialysis Tubing Potassium Acetate Polypropylene Centrifuge Tube Supercoiled Plasmid Insertion Inactivation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. 1.
    Clewell, D.B. and Helinski, D.R. (1971) Properties of a super-coiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry 9, 4428–4440.Google Scholar

Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • Stephen A. Boffey
    • 1
  1. 1.Division of Biological and Environmental StudiesThe Hatfield PolytechnicUK

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