Protein (Western) Blotting with Immunodetection
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Rapid staining/destaining: once transferred, staining and destaining can be achieved within 5 min.
There is no ampholyte staining when analyzing IEF gels.
Samples in preparative gels may be located rapidly by briefly blotting the gel.
Low concentrations of samples can be detected since they are not spread throughout the thickness of a gel, but “concentrated” at the surface of the paper. As a consequence, it is possible to use lower specific activities and lower concentrations of expensive or scarce probes.
The “blot” is a convenient permanent record of the gel run.
KeywordsScalpel Blade Lower Specific Activity Protein Blotting Nitrocellulose Sheet Nitrocellulose Paper
- Notes on Immunoblotting. A free eight-page booklet, Mercia Brocades, Surrey, England.Google Scholar