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SDS Polyacrylamide Gel Electrophoresis of Proteins

Determination of Protein Molecular Weights and High-Resolution Silver Staining
  • John M. Walker
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Polyacrylamide gel electrophoresis (PAGE) is a quick and sensitive method for analyzing the composition of mixtures of proteins. Since the early 1970s, this method has become a routine and frequently used analytical procedure in all protein chemistry laboratories, and as such, biology students should of course be familiar with this basic technique. Originally gels were formed in small glass tubes and one sample loaded on each gel, but between-run variation made it difficult to directly compare one sample with another. This problem was overcome by the introduction of the slab gel system (1), in which a large number of samples can be compared on a single run. It is this system that is described in this experiment.

Keywords

Silver Nitrate Ammonium Persulfate Refractive Index Change Unknown Protein Acrylamide Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.PubMedCrossRefGoogle Scholar
  2. 2.
    Weber, K. and Osborne, M. (1969) The reliability of molecular weight determinations by sodium dodecyl sulfate polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406.PubMedGoogle Scholar
  3. 3.
    Morrissey, J. (1981) Silver stain for proteins in polyacrylamide gels. A modified procedure with enhanced uniform sensitivity. Anal. Biochem. 117, 307–310.PubMedCrossRefGoogle Scholar
  4. 4.
    Studier, F. W. (1973) Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J. Mol. Biol. 79, 237–248.PubMedCrossRefGoogle Scholar

Further Reading

  1. Smith, I. (1976) Zone Electrophoresis, Chromatographic, and Electrophoretic Techniques, Vol. 2, Heinemann Medical Books, London.Google Scholar
  2. Smith, B. J. and Nicholas, R. H. (1983) Analytical Electrophoretic Techniques in Protein Chemistry, in Techniques in Molecular Biology (J. M. Walker and W. Gaastra, eds.) Croom Helm, Beckenham, Kent, England.Google Scholar

Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • John M. Walker
    • 1
  1. 1.Division of Biological and Environmental StudiesThe Hatfield PolytechnicUK

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