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Laser Microdissection

  • Graeme I. Murray
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Solid tissues and organs, especially diseased tissues, are complex structures composed of heterogeneous mixtures of morphologically and functionally distinct cell types. The purposeful molecular study of cytologically and/or phenotypically specific cell types from tissues either normal or often more importantly abnormal or diseased requires the availability of rapid, efficient and accurate methods for obtaining specific defined groups of cells for further study. Whereas for example circulating blood cells can readily be separated into their distinct morphological and phenotypic classes by cell sorting using flow cytometry obtaining specific types of cells from solid tissues for analysis has until recently been much more difficult. Although manual methods of tissue microdissection have been described they are slow, cumbersome and not very specific. However, the development of laser based methods of microdissection for selecting specific types of cells from thin sections...

Keywords

Laser Capture Microdissection Fixed Tissue Plastic Membrane Microdissected Tissue Unfixed Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Graeme I. Murray
    • 1
  1. 1.Department of PathologyUniversity Medical Buildings, University of AberdeenAberdeenUK

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