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Cryopreservation

Conservation of Bioresources at Ultra Low Temperatures
  • John G. Day
  • Keith C. Harding
  • Jayanthi Nadarajan
  • Erica E. Benson
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Summary

Cryopreservation is the ultra-low temperature storage (usually in liquid nitrogen at ca. -135 to -196°C) of living cells, tissues and organs capable of resuming normal functions after retrieval from a cryobank. This chapter explains the basic principles of cryopreservation with respect to strategies currently used to cryoprotect the diverse biological materials held in cryogenic storage. Approaches used to minimize or obviate the lethal and sublethal effects of cryoinjury are particularly highlighted. Examples of how cryopreservation has been universally applied to safeguard and preserve the wide spectrum of biological resources used in agriculture, biotechnology, healthcare, and biodiversity conservation are summarized and general guidance is offered for the management of bioresources in cryobanks.

Keywords

Biological resource center cryoinjury cryopreservation vitrification. 

Notes

Acknowledgments

The authors acknowledge the European Union's 5th Framework Programme, Quality of Life and Management of Living Resources, Research Infrastructures Biological Collections, COBRA Project QLRT-2000-01645 and CRYMCEPT Project (QLK5-CT-2002-01279). J Nadarajan acknowledges the European Social Fund, The Forest Research Institute of Malaysia and the University of Abertay Dundee for the support of her postgraduate studies.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • John G. Day
    • 1
  • Keith C. Harding
    • 2
  • Jayanthi Nadarajan
    • 3
  • Erica E. Benson
    • 4
  1. 1.Curator CCAP, Culture Collection of Algae and Protozoa, Scottish Association for Marine ScienceDunstaffnage Marine LaboratoryScotlandUK
  2. 2.Environmental Science and BiotechnologyScotlandUK
  3. 3.Millennium Seed BankRoyal Botanic Gardens, Ardingly, Haywards HeathUK
  4. 4.Environmental Science and BiotechnologyScotlandUK

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