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Southern Blotting as a Diagnostic Method

  • Bronwen M. Harvey
  • Pirkko Soundy
Protocol
  • 3.1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Nucleic acid hybridization is a process in which complementary single strands of nucleic acids combine to achieve a stable double-stranded nucleic acid molecule. This action has been used to establish a molecular or genetic relatedness among organisms and to characterize their genomes. Furthermore, this technique is one of several diagnostic tools useful for detecting a wide variety of conditions. Since the determination of the basic principles of duplex formation and stability in the 1950s, many variations of the hybridization techniques have been developed. Southern first transferred DNA fragments from agarose, after electrophoretic separation, onto nitrocellulose (1). The technique is known as Southern blotting. Alwine et al (2) described shortly afterward a similar Northern blotting technique in which separated RNA strands are transferred from an agarose gel to a suitable solid support. A logical extension of these blotting techniques has been the dot or slot blot...

Keywords

Southern Blotting Sickle Cell Anemia Acute Promyelocytic Leukemia Duchenne Muscular Dystrophy FMR1 Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bronwen M. Harvey
    • 1
  • Pirkko Soundy
    • 2
  1. 1.GE Healthcare, Medical DiagnosticsGroup CentreAmersham, BuckinghamshireUK
  2. 2.GE HealthcareThe Group CentreAmersham, BuckinghamshireUK

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