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Amino Acid Analysis

  • Ian Davidson
  • Paula O'Connor
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Amino acid analysis is carried out to provide information on the different types and concentrations of amino acids in proteins and peptides from both natural and synthetic sources. It is also an important analysis technique in the determination of levels of free amino acids in foodstuffs and clinical specimens such as physiological fluids and tissues. In addition to the 20 well known amino acids some 700 unusual naturally occurring amino acids have been reported (1,2).

The identification and quantification of amino acids can be divided into five constituent parts, sample purification and preparation, hydrolysis, derivatization, analysis, and data handling.
  1. a)

    Sample purification and preparation:In many types of samples the amino acids can be found in media not compatible with any of the analytical systems available. The presence of buffer salts, urea, detergents etc., can interfere with both the hydrolysis and subsequent derivatization so some purification and sample...

Keywords

Free Amino Acid Amino Acid Analysis Amino Acid Derivative Reverse Phase Liquid Chromatography Dansyl Chloride 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Ian Davidson
    • 1
  • Paula O'Connor
    • 1
  1. 1.School of Medical SciencesUniversity of Medical Sciences, IMS BuildingForesterhillScotland, UK

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