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Probe Design, Production, and Applications

  • Marilena Aquino de Muro
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

A probe is a nucleic acid molecule (single-stranded DNA or RNA) with a strong affinity with a specific target (DNA or RNA sequence). Probe and target base sequences must be complementary to each other, but depending on conditions, they do not necessarily have to be exactly complementary. The hybrid (probe–target combination) can be revealed when appropriate labeling and detection systems are used. Gene probes are used in various blotting and in situ techniques for the detection of nucleic acid sequences. In medicine, they can help in the identification of microorganisms and the diagnosis of infectious, inherited, and other diseases.

2. Probe Design

The probe design depends on whether a gene probe or an oligonucleotide probe is desired.

2.1. Gene Probes

Gene probes are generally longer than 500 bases and comprise all or most of a target gene. They can be generated in two ways. Cloned probes are normally used when a specific clone is available or when the DNA sequence is...

Keywords

Oligonucleotide Probe Gene Probe Nucleic Acid Sequence Nick Translation Polymerase Chain Reaction Cycle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Marilena Aquino de Muro
    • 1
  1. 1.University of Caxias of the South, Francisco Getulio VargasPetropolis, Caxias of the SouthBrazil

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