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Mass Spectrometry of Proteins and Peptides

  • Kenneth G. Standing
Protocol
  • 3k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

In the last few years, mass spectrometry (MS) has emerged as a major tool for the identification and characterization of peptides and proteins. It is now possible to measure proteins of masses > 100 kDa to an accuracy of a few Da, and to measure the masses of peptides with an accuracy of a few mDa. As a result, the information yielded by an MS measurement is highly specific. In addition, MS has extremely high sensitivity, often in the femtomole (10−15 mole) range, so it is a method that is suitable for the analysis of trace amounts of sample.

Mass spectrometry relies on the properties of charged particles moving under the influence of electric and magnetic fields, so the species studied must be ions (charge ze) rather than molecules. Most MS experiments are performed on positive ions, which can be formed either by the removal of one or more electrons from each molecule, or by the addition of one or more cations, usually protons. Consequently, the first step in any mass...

Keywords

Quadrupole Mass Filter Magnetic Sector Mass Spectrometer Pulse Laser Fire Linear Quadrupole Mass Filter Symmetric Electrostatic Field 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Kenneth G. Standing
    • 1
  1. 1.Department of Physics and AstronomyUniversity of ManitobaWinnipeg, ManitobaCanada

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