Autoradiography and Fluorography

  • Bronwen M. Harvey
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Autoradiography is a technique widely used in biology and medicine. It uses a photographic emulsion to visualize molecules that are radioactively labeled. The technique provides a convenient method of detection, giving an accurate representation of the distribution of the label within a sample. The image is often referred to as the autoradiograph or autoradiogram.

The image is generated through the radioactive decay of the labeled ligand that is bound to a protein and/or nucleic acid found within a tissue or cell or held on an insert solid support (see Table 26.1). For example, it can be used to:
  • Identify a specific protein from a mixture separated by polyacrylamide electrophoresis (1) and held on a support: Western blotting (2).

  • Identify nucleic acid fragments separated by gel electrophoresis held on a support: Southern blotting (3), Northern blotting (4).

  • Identify the distribution of nucleic acids in a tissue section: in situ hybridization (5,6).

  • Identify the cellular...


Photographic Emulsion Latent Image Silver Atom Silver Halide Silver Iodide 
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Figures reproduced from a “Guide to Autoradiography” and Efficient detection of Biomolecules by Autoradiography, Fluorography and Chemiluminescence. Review booklet 23. GE Healthcare UK Ltd, Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, England.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bronwen M. Harvey
    • 1
  1. 1.GE HealthcareMedical Diagnostics, Group CentreAmershamUK

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