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Capillary Electrophoresis of Proteins

  • Mark Strege
Protocol
  • 3.1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

1.1. Capillary Electrophoresis of Proteins

Capillary electrophoresis (CE) is a separation technique that combines aspects of both gel electrophoresis and high performance liquid chromatography (HPLC). As is the case for gel electrophoresis, the separation in CE is based upon differential migration in an electrical field. Like HPLC, the detection of the migrating sample analytes may be monitored on-line or postcolumn/capil-lary for both quantitation and characterization, thereby eliminating the need for labor-intensive staining and destaining. The CE separation may take place within free solution or in the presence of a viscous gel medium. Since its initial description by Hjerten in 1967 (1), CE techniques analogous to most conventional electrophoretic methods have been demonstrated, including zone electrophoresis, displacement electrophoresis (isotachophoresis), isoelectric focusing, and molecular sieving separations. Data presentation and analysis are also similar to...

Keywords

High Performance Liquid Chromatography Capillary Electrophoresis Carbohydrate Chain Capillary Electrophoresis Separation Biopharmaceutical Industry 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Mark Strege
    • 1
  1. 1.Lilly Research Laboratories, Eli Lilly and Co.Lilly Corporate CenterIndianapolisIN

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