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Staining of Glycoproteins/Proteoglycans on SDS-Gels

  • Holger J. Møller
  • Jørgen H. Poulsen
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of mol-wt determinations. In this chapter, staining methods will be described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.

Keywords

Alcian Blue Periodic Acid Development Step Silver Solution Automatic Staining 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Holger J. Møller
    • 1
  • Jørgen H. Poulsen
    • 1
  1. 1.Department of Clinical BiochemistryKH University Hospital, NørrebrogadeÅrhusDenmark

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