Staining of Glycoproteins/Proteoglycans on SDS-Gels

  • Holger J. Møller
  • Jørgen H. Poulsen
Part of the Springer Protocols Handbooks book series (SPH)


Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is a commonly employed technique for separation of proteins according to size. Among other applications, it is used for identification and characterization of proteins on the basis of mol-wt determinations. In this chapter, staining methods will be described that permit detection of highly glycosylated proteins on SDS gels at levels of a few nanograms.


Alcian Blue Periodic Acid Development Step Silver Solution Automatic Staining 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Holger J. Møller
    • 1
  • Jørgen H. Poulsen
    • 1
  1. 1.Department of Clinical BiochemistryKH University Hospital, NørrebrogadeÅrhusDenmark

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