C-Terminal Sequence Analysis with Carboxypeptidase Y

  • John M. Walker
  • Julia S. Winder
Part of the Springer Protocols Handbooks book series (SPH)


To date there is no chemical method that provides the ability to determine extensive lengths of amino acid sequence sequentially at the C-terminus of a protein or a peptide, although the thiocyanate method described in Chapter 92 is showing great promise following recent developments (extensive sequence data from the N-terminus can of course be achieved using the Edman degradation). However, limited C-terminal sequence data can be obtained fairly quickly and easily using a class of enzymes called carboxypeptidases. Such limited information can be useful, for example, in determining positions of proteolytic cleavage in proteins, or for confirming sequence data toward the end of a peptide or protein sequence.


Free Amino Acid Amino Acid Analysis Edman Degradation Saturated Ammonium Sulfate Thiocyanate Method 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • John M. Walker
    • 1
  • Julia S. Winder
    • 1
  1. 1.Division of BiosciencesUniversity of HertfordshireHatfieldUK

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