Amino Acid Analysis Using Precolumn Derivatization with Phenylisothiocyanate

  • G. Brent Irvine
Part of the Springer Protocols Handbooks book series (SPH)


The method described in this chapter is based on derivatization of amino acids, produced by hydrolysis of peptides or proteins, with phenylisothiocyanate. This forms phenylthiocarbamyl amino acids, which are then separated by reversed-phase high-performance liquid chromatography and quantified from their UV absorbance at 254 nm.


Cold Trap Sodium Dihydrogen Phosphate Methanesulfonic Acid Amino Acid Standard Dansyl Chloride 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • G. Brent Irvine
    • 1
  1. 1.School of Biology and BiochemistryQueen’s University of BelfastIreland

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