Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

  • Ralph C. Judd
Part of the Springer Protocols Handbooks book series (SPH)


The comparison of the primary structure of proteins is an important facet in the characterization of families of proteins from the same organism, similar proteins from different organisms, and cloned gene products. There are many methods available to establish the sequence similarities of proteins. A relatively uncomplicated approach is to compare the peptide fragments of proteins generated by enzymatic or chemical cleavage, i.e., peptide mapping. The similarity or dissimilarity of the resultant peptides reflects the similarity or dissimilarity of the parent proteins.


Peptide Fragment Razor Blade Coomassie Brilliant Blue Parent Protein Resultant Peptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Ralph C. Judd
    • 1
  1. 1.Division of Biological SciencesUniversity of MontanaMissoula

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