Advertisement

Peptide Mapping by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis

  • Ralph C. Judd
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The comparison of the primary structure of proteins is an important facet in the characterization of families of proteins from the same organism, similar proteins from different organisms, and cloned gene products. There are many methods available to establish the sequence similarities of proteins. A relatively uncomplicated approach is to compare the peptide fragments of proteins generated by enzymatic or chemical cleavage, i.e., peptide mapping. The similarity or dissimilarity of the resultant peptides reflects the similarity or dissimilarity of the parent proteins.

Keywords

Peptide Fragment Razor Blade Coomassie Brilliant Blue Parent Protein Resultant Peptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Cleveland, D. W., Fischer, S. G., Kirschner, M. W., and Laemmli, U. K. (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J. Biol. Chem. 252, 1102–1106.PubMedGoogle Scholar
  2. 2.
    Judd, R. C. (1986) Evidence for N-terminal exposure of the PIA subclass of protein I of Neisseria gonorrhoeae. Infect. Immunol. 54, 408–414.Google Scholar
  3. 3.
    Judd, R. C. (1987) Radioiodination and 125I-labeled peptide mapping on nitrocellulose membranes. Anal. Biochem. 160, 306–315.PubMedCrossRefGoogle Scholar
  4. 4.
    Moos, M., Jr. and Nguyen, N. Y. (1988) Reproducible high-yield sequencing of proteins electrophoretically separated and transferred to an inert support. J. Biol. Chem. 263, 6005–6008.PubMedGoogle Scholar
  5. 5.
    Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–695.PubMedCrossRefGoogle Scholar
  6. 6.
    Caldwell, H. D. and Judd, R. C. (1982) Structural analysis of chlamydial proteins. Infect. Immunol. 38, 960–968.Google Scholar
  7. 7.
    Schagger, H. and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166, 368–397.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Ralph C. Judd
    • 1
  1. 1.Division of Biological SciencesUniversity of MontanaMissoula

Personalised recommendations