Protein Quantitation Using Flow Cytometry

  • F. Andrew Ray
  • Thomas D. Friedrich
  • Judith Laffin
  • John M. Lehman
Part of the Springer Protocols Handbooks book series (SPH)


It is often desirable to know how much of a particular protein is produced by a particular cell. For example, is this protein expressed at low levels throughout a population of cells, or is this protein expressed at very high levels in some cells and not in others? Does the expression of the protein change in a particular cell-cycle compartment? Technical protocols that require cell lysis, such as immunoblotting (1), allow the mean expression level of a given protein to be compared between populations of cells. It is even possible to use the immunoblotting protocol to determine the amount of a given protein expressed by a population of cells, if the protein has been purified for use as a standard. However, cell lysis obliterates the potential for single-cell quantitation of proteins.


Propidium Iodide Repeat Centrifugation Flow Cytometer System Target Protein Molecule Minimize Cell Loss 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • F. Andrew Ray
    • 1
  • Thomas D. Friedrich
    • 1
  • Judith Laffin
    • 1
  • John M. Lehman
    • 1
  1. 1.Department of Microbiology, Immunology, and Molecular GeneticsThe Albany Medical CollegeAlbany

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