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Detection of Proteins on Blots Using Chemiluminescent Systems

  • Graeme R. Guy
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The detection of membrane-bound antibodies is normally carried out using either radioactive labeled secondary antibody (or protein A) or enzyme conjugates of an appropriate secondary antibody followed by an enzyme-catalyzed color reaction. These detection systems, however, may be either hazardous, time-consuming, or lacking in sensitivity. In addition, colors tend to fade with time and exposure to light.

Keywords

Enzyme Conjugate Dihydrogen Orthophosphate Primary Blocking Enhance Chemiluminescence Western Blot Aqueous H2O2 Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Gunderman, K. D. and McCapra, F. (1987) Chemiluminescence in Organic Chemistry. Springer-Verlag, Berlin, Germany, pp. 77–108.Google Scholar
  2. 2.
    Thorpe, G. H. G., Kricka, L. J., Moseley, M. R., and Whitehead, T. P. (1985) Phenols as enhancers of the chemiluminescent horse radish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays. Clin. Chem. 31, 1335–1341.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Graeme R. Guy
    • 1
  1. 1.Institute of Molecular and Cell BiologyNational University of SingaporeSingapore

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