Detection of Proteins on Blots Using Chemiluminescent Systems

  • Graeme R. Guy
Part of the Springer Protocols Handbooks book series (SPH)


The detection of membrane-bound antibodies is normally carried out using either radioactive labeled secondary antibody (or protein A) or enzyme conjugates of an appropriate secondary antibody followed by an enzyme-catalyzed color reaction. These detection systems, however, may be either hazardous, time-consuming, or lacking in sensitivity. In addition, colors tend to fade with time and exposure to light.


Enzyme Conjugate Dihydrogen Orthophosphate Primary Blocking Enhance Chemiluminescence Western Blot Aqueous H2O2 Solution 
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    Gunderman, K. D. and McCapra, F. (1987) Chemiluminescence in Organic Chemistry. Springer-Verlag, Berlin, Germany, pp. 77–108.Google Scholar
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    Thorpe, G. H. G., Kricka, L. J., Moseley, M. R., and Whitehead, T. P. (1985) Phenols as enhancers of the chemiluminescent horse radish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays. Clin. Chem. 31, 1335–1341.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Graeme R. Guy
    • 1
  1. 1.Institute of Molecular and Cell BiologyNational University of SingaporeSingapore

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