Detection of Proteins on Blots Using Chemiluminescent Systems
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The detection of membrane-bound antibodies is normally carried out using either radioactive labeled secondary antibody (or protein A) or enzyme conjugates of an appropriate secondary antibody followed by an enzyme-catalyzed color reaction. These detection systems, however, may be either hazardous, time-consuming, or lacking in sensitivity. In addition, colors tend to fade with time and exposure to light.
KeywordsEnzyme Conjugate Dihydrogen Orthophosphate Primary Blocking Enhance Chemiluminescence Western Blot Aqueous H2O2 Solution
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