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Detection of Proteins on Blots Using Avidin- or Streptavidin-Biotin

  • William J. LaRochelle
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Since the initial publication by Towbin and coworkers (1) on the preparation of replicas of sodium dodecyl sulfate(SDS)-polyacrylamide gel patterns, commonly called protein blots, the technique of transferring proteins from inaccessible gel matrices to accessible solid supports, such as nitrocellulose or nylon membranes, has become widely utilized (2,3).

Keywords

Sodium Dodecyl Sulfate Sodium Dodecyl Sulfate Horseradish Peroxidase Wash Solution Newborn Calf Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • William J. LaRochelle
    • 1
  1. 1.Laboratory of Cellular and Molecular BiologyNational Cancer Institute, National Institutes of HealthBethesda

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