Fluorescent Protein Staining on Nitrocellulose with Subsequent Immunodetection of Antigen

  • Donald F. Summers
  • Boguslaw Szewczyk
Part of the Springer Protocols Handbooks book series (SPH)


The transfer of proteins from gels to nitrocellulose or other immobilizing matrices has become increasingly popular as a powerful tool for the subsequent analysis of proteins. Most frequently, the blotted proteins are analyzed for their antigenic properties by Western blotting.


Aluminium Foil Fluorescent Staining Protein Staining Antigenic Property Blot Protein 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Donald F. Summers
    • 1
  • Boguslaw Szewczyk
    • 1
  1. 1.Department of Microbiology and Molecular Genetics, College of MedicineUniversity of CaliforniaIrvine

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