Horseradish Peroxidase Labeling of Antibody Using Periodate Oxidation
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The most commonly used method (1) for labeling IgG antibody molecules with horseradish peroxidase exploits the glycoprotein nature of the enzyme. The saccharide residues are oxidized with sodium periodate to produce aldehyde groups that can react with the amino groups of the IgG molecule, and the Schiff bases formed are then reduced to give a stable conjugate of high molecular weight (0.5–1 × 106). The peroxidase has few free amino groups, so self-coupling is not a significant problem.
KeywordsHorseradish Peroxidase Schiff Base Sodium Acetate Buffer Sodium Borohydride Sodium Periodate
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