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β-Galactosidase Labeling of Antibody Using MBS

  • G. Brian Wisdom
Protocol
  • 62 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The heterobifunctional reagent, m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), is of value when one of the components of a conjugate has no free thiol groups, e.g., IgG. In this method (1), the IgG antibody is first modified by allowing the N-hydroxysuccinimide ester group of the MBS to react with amino groups in the IgG; the β-galactosidase is then added and the maleimide groups on the modified IgG react with thiol groups in the β-galactosidase to form thioether links. This procedure produces conjugates with molecular weights in the range 0.6–1 × 106.

Keywords

Bovine Serum Albumin Sodium Phosphate Buffer Microbial Growth Thiol Group Free Thiol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • G. Brian Wisdom
    • 1
  1. 1.Division of Biochemistry, School of Biology and BiochemistryThe Queen’s University, Medical Biology CentreBelfastUK

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