β-Galactosidase Labeling of Antibody Using MBS
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The heterobifunctional reagent, m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS), is of value when one of the components of a conjugate has no free thiol groups, e.g., IgG. In this method (1), the IgG antibody is first modified by allowing the N-hydroxysuccinimide ester group of the MBS to react with amino groups in the IgG; the β-galactosidase is then added and the maleimide groups on the modified IgG react with thiol groups in the β-galactosidase to form thioether links. This procedure produces conjugates with molecular weights in the range 0.6–1 × 106.
KeywordsBovine Serum Albumin Sodium Phosphate Buffer Microbial Growth Thiol Group Free Thiol
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