Drying Polyacrylamide Gels
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Gels can be fragile and difficult to handle and store without suffering damage. This can be overcome by drying, whereby they become bonded to a stabilizing medium. Furthermore, it may be necessary to dry a gel in order to obtain the most efficient detection of radioactive samples on it by autoradiography, and fluorography. In this chapter, two methods are described—the first being drying onto absorbent paper (this being suitable for storage, reflection densitometry, and autoradiography), and the second being drying between cellulose sheets (this being suitable for storage, transmission densitometry, and demonstration by overhead projection). Both methods are suitable for slab gels—tube-shaped gels are not suited to drying down.
KeywordsSilicon Rubber Polyethylene Sheet Rubber Sheet Absorbent Paper Overhead Projection
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