Zn2+-Reverse Staining of Proteins in Polyacrylamide Gels

  • Juan M. García-Segura
  • Mercedes Ferreras
Part of the Springer Protocols Handbooks book series (SPH)


Unlike the traditional methods for staining of polyacrylamide gels (e.g., Coomassie blue, silver stain) whose purpose is to dye the protein bands (1,2), the purpose of reverse staining (RS) methods is to leave protein bands unstained and dye the rest of the gel instead. RS methods are usually based on the precipitation of some metal salt all along the gel, except in the positions where proteins are. The contrast between protein bands and background, and the sensitivity of a particular RS method, will depend on both the intensity of the selective precipitation and the color of the precipitate.


Protein Band Electrophoretic Pattern Coomassie Blue Zinc Sulfate Deep Blue 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Juan M. García-Segura
    • 1
  • Mercedes Ferreras
    • 1
  1. 1.Departimiento de Bioquímica y Biología MolecularUniversidad ComplutenseMadridSpain

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