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Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension

  • Patricia Gravel
  • Olivier Golaz
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is one of the most powerful tools for the separation of complex protein mixtures. This method separates individual proteins and polypeptide chains according to their isoelectric point and molecular weight. This chapter describes the protocol for 2D PAGE using carrier ampholyte pH gradient gel for the isoelectric focusing (IEF) separation (pH gradient 3.5–10.0) and a polyacrylamide gradient gel for the second dimension (1,2).

Keywords

Sodium Dodecyl Sulfate Capillary Tube Lysis Solution Cathodic Side Glass Test Tube 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Patricia Gravel
    • 1
  • Olivier Golaz
    • 2
  1. 1.Clinical Research UnitPsychiatric Institutions of GenevaSwitzerland
  2. 2.Central Clinical Chemistry LaboratoryUniversity Hospital of GenevaSwitzerland

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