Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension

  • Patricia Gravel
  • Olivier Golaz
Part of the Springer Protocols Handbooks book series (SPH)


Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is one of the most powerful tools for the separation of complex protein mixtures. This method separates individual proteins and polypeptide chains according to their isoelectric point and molecular weight. This chapter describes the protocol for 2D PAGE using carrier ampholyte pH gradient gel for the isoelectric focusing (IEF) separation (pH gradient 3.5–10.0) and a polyacrylamide gradient gel for the second dimension (1,2).


Sodium Dodecyl Sulfate Capillary Tube Lysis Solution Cathodic Side Glass Test Tube 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Patricia Gravel
    • 1
  • Olivier Golaz
    • 2
  1. 1.Clinical Research UnitPsychiatric Institutions of GenevaSwitzerland
  2. 2.Central Clinical Chemistry LaboratoryUniversity Hospital of GenevaSwitzerland

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