Two-Dimensional PAGE Using Carrier Ampholyte pH Gradients in the First Dimension
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Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is one of the most powerful tools for the separation of complex protein mixtures. This method separates individual proteins and polypeptide chains according to their isoelectric point and molecular weight. This chapter describes the protocol for 2D PAGE using carrier ampholyte pH gradient gel for the isoelectric focusing (IEF) separation (pH gradient 3.5–10.0) and a polyacrylamide gradient gel for the second dimension (1,2).
KeywordsSodium Dodecyl Sulfate Capillary Tube Lysis Solution Cathodic Side Glass Test Tube
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