SDS-Polyacrylamide Gel Electrophoresis of Peptides

  • Ralph C. Judd
Part of the Springer Protocols Handbooks book series (SPH)


Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) has proven to be among the most useful tools yet developed in the area of molecular biology. The discontinuous buffer system, first described by Laemmli (1), has made it possible to separate, visualize, and compare readily the component parts of complex mixtures of molecules (e.g., tissues, cells). SDS-PAGE separation of proteins and peptides makes it possible to quantify the amount of a particular protein/peptide in a sample, obtain fairly reliable molecular mass information, and, by combining SDS-PAGE with immunoelectroblotting, evaluate the antigenicity of proteins and peptides. SDS-PAGE is both a powerful separation system and a reliable preparative purification technique (2 and see Chapter 11).


Ammonium Persulfate Coomassie Brilliant Blue Back Plate Front Plate Discontinuous Buffer System 
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Ralph C. Judd
    • 1
  1. 1.Division of Biological SciencesUniversity of MontanaMissoula

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