Screening Hybridoma Culture Supernatants Using ELISA
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Enzyme-linked immunosorbent assay (ELISA) is a widely used method for the detection of antibody and is appropriate for use for screening hybridoma supernatants (1). As with radiobinding assays, it is a solid-phase binding assay that is quick, reliable, and accurate. The method is often preferred to radioactive assays, since the handling and disposal of radioisotopes is avoided and the enzyme conjugates are more stable than radioiodinated proteins. However, most of the substrates for the enzyme reactions are carcinogenic or toxic and, hence, require handling with care.
KeywordsMagnesium Chloride Carbonate Buffer Phosphate Solution Enzyme Conjugate Radioactive Assay
- 1.Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed. Blackwell Science, Oxford, UK.Google Scholar