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Screening Hybridoma Culture Supernatants Using ELISA

  • Mark Page
  • Robin Thorpe
Protocol
  • 64 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Enzyme-linked immunosorbent assay (ELISA) is a widely used method for the detection of antibody and is appropriate for use for screening hybridoma supernatants (1). As with radiobinding assays, it is a solid-phase binding assay that is quick, reliable, and accurate. The method is often preferred to radioactive assays, since the handling and disposal of radioisotopes is avoided and the enzyme conjugates are more stable than radioiodinated proteins. However, most of the substrates for the enzyme reactions are carcinogenic or toxic and, hence, require handling with care.

Keywords

Magnesium Chloride Carbonate Buffer Phosphate Solution Enzyme Conjugate Radioactive Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. 1.
    Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed. Blackwell Science, Oxford, UK.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.MEDEVA, Vaccine Research Unit, Department of BiochemistryImperial College of Science, Technology, and MedicineLondonUK
  2. 2.National Institute for Biological Standards and ControlPotters BarUK

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