Screening Hybridoma Culture Supernatants Using ELISA

  • Mark Page
  • Robin Thorpe
Part of the Springer Protocols Handbooks book series (SPH)


Enzyme-linked immunosorbent assay (ELISA) is a widely used method for the detection of antibody and is appropriate for use for screening hybridoma supernatants (1). As with radiobinding assays, it is a solid-phase binding assay that is quick, reliable, and accurate. The method is often preferred to radioactive assays, since the handling and disposal of radioisotopes is avoided and the enzyme conjugates are more stable than radioiodinated proteins. However, most of the substrates for the enzyme reactions are carcinogenic or toxic and, hence, require handling with care.


Magnesium Chloride Carbonate Buffer Phosphate Solution Enzyme Conjugate Radioactive Assay 
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  1. 1.
    Johnstone, A. and Thorpe, R. (1996) Immunochemistry in Practice, 3rd ed. Blackwell Science, Oxford, UK.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Mark Page
    • 1
  • Robin Thorpe
    • 2
  1. 1.MEDEVA, Vaccine Research Unit, Department of BiochemistryImperial College of Science, Technology, and MedicineLondonUK
  2. 2.National Institute for Biological Standards and ControlPotters BarUK

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