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Production of Highly Specific Polyclonal Antibodies Using a Combination of 2D Electrophoresis and Nitrocellulose-Bound Antigen

  • Monique Diano
  • André Le Bivic
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Highly specific antibodies directed against minor proteins, present in small amounts in biological fluids, or against nonsoluble cytoplasmic or membraneous proteins, are often difficult to obtain. The main reasons for this are the small amounts of protein available after the various classical purification processes and the low purity of the proteins.

Keywords

Antigenic Protein Polyclonal Antiserum Irrelevant Antibody Large Loading Capacity Specific Polyclonal Antiserum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Monique Diano
    • 1
  • André Le Bivic
    • 1
  1. 1.Lab de GenetiqueCNRSMarseilleFrance

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