Raising of Polyclonal Antisera

  • Graham S. Bailey
Part of the Springer Protocols Handbooks book series (SPH)


Suitable antisera are essential for use in all immunochemical procedures. Three important properties of an antiserum are avidity, specificity, and titer. The avidity of an antiserum is a measure of the strength of the interactions of its antibodies with an antigen. The specificity of an antiserum is a measure of the ability of its antibodies to distinguish the immunogen from related antigens. The titer of an antiserum is the final (optimal) dilution at which it is employed in the procedure; it depends on the concentrations of the antibodies present and on their affinities for the antigen. The values of those parameters required for a particular antiserum very much depend on the usage to which the antiserum will be put. For example, for use in radioimmunoassay, it is best to have a monospecific antiserum of high avidity, whereas for use in immunoaffinity chromatography, the monospecific antiserum should not possess too high an avidity. Otherwise, it may prove impossible to elute the desired antigen without extensive denaturation.


Polyclonal Antiserum Glass Syringe Antibody Molecule Booster Injection High Avidity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Hum, B. A. L. and Chantler, S. M. (1980) in Production of Reagent Antibodies in Methods in Enzymology, vol. 70 (Van Vunakis, H. and Langone, J. J., eds.), Academic, New York, pp. 104–141.Google Scholar
  2. 2.
    Dresser, D. W. (1986) Immunization of experimental animals, in Handbook of Experimental Immunology, vol. 1 (Weir, D. M., Herzenberg, L. A., Blackwell, C., and Herzenberg, L. A., eds.), 4th ed. Blackwell, Oxford, UK, pp. 8.1–8.21.Google Scholar
  3. 3.
    Chard, T. (1987) Requirements for binding assays-antibodies and other binders, in Laboratory Techniques in Biochemistry and Molecular Biology, vol. 6, part 2, 3rd rev. ed. Elsevier, Amsterdam, pp. 88–110.Google Scholar
  4. 4.
    Vaitukaitis, J. L. (1981) Production of antisera with small doses of immunogen: multiple intradermal injections, in Methods in Enzymology, vol. 73 (Langone, J. J. and Van Vunakis, H., eds.), Academic, New York, pp. 46–57.Google Scholar
  5. 5.
    Al-Hamidi, A. A. A. and Bailey, G. S. (1991) Purification of prokallikrein from bovine pancreas. Biochim. Biophys. Acta 1075, 88–92.PubMedGoogle Scholar
  6. 6.
    Bailey, G. S. (1994) Radioimmunoassay of peptides and proteins, in Methods in Molecular Biology, vol. 32 (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 449–459.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Graham S. Bailey
    • 1
  1. 1.Department of Biological and Chemical SciencesUniversity of EssexColchesterUK

Personalised recommendations