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Conjugation of Peptide to Carrier Proteins via m-Maleimidobenzoyl-N-Hydroxysuccinimide Ester (MBS)

  • J. Mark Carter
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Abstract

m-Maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) is a heterobifunctional agent that links a thiol group to an amino group at neutral pH. For peptide conjugation, the peptide usually provides the thiol group in the form of a cysteine residue, whereas the carrier provides amino groups in the form of lysine residues. The reaction proceeds in two steps. First the carrier is activated by reaction of its amino group with the succinimide moiety. Then the thiol group on the peptide reacts with the maleimide moiety of the activated carrier.

Keywords

Thiol Group Keyhole Limpet Hemocyanin Peptide Conjugation Cysteine Thiol Overnight Dialysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • J. Mark Carter
    • 1
  1. 1.CytogenPrinceton

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