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The Chloramine T Method for Radiolabeling Protein

  • Graham S. Bailey
Protocol
  • 70 Downloads
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Many different substances can be labeled by radioiodination. Such labeled molecules are of major importance in a variety of investigations, e.g., studies of intermediary metabolism, determinations of agonist and antagonist binding to receptors, quantitative measurements of physiologically active molecules in tissues and biological fluids, and so forth. In most of those studies, it is necessary to measure very low concentrations of the particular substance, and that in turn, implies that it is essential to produce a radioactively labeled tracer molecule of high specific radioactivity. Such tracers, particularly in the case of proteins, can often be conveniently produced by radioiodination.

Keywords

Sodium Phosphate Buffer Radioactive Iodine Isotopic Abundance Potassium Iodide Intermediary Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Bolton, A. E. (1985) Radioiodination Techniques, 2nd ed. Amersham International, Amersham, Bucks, UK.Google Scholar
  2. 2.
    Bailey, G. S. (1990) In vitro labeling of proteins, in Radioisotopes in Biology (Slater, R. J., ed.), IRL, Oxford, UK, pp. 191–205.Google Scholar
  3. 3.
    Hunter, W. M. and Greenwood, F. C. (1962) Preparation of iodine-131 labeled human growth hormone of high specific activity, Nature 194, 495,496.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Graham S. Bailey
    • 1
  1. 1.Department of Biological and Chemical SciencesUniversity of EssexColchesterUK

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