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N-Linked Oligosaccharide Profiling by HPLC on Porous Graphitized Carbon (PGC)

  • Elizabeth F. Hounsell
  • Michael J. Davies
  • Kevin D. Smith
Protocol
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Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The vast array of possible N-linked oligosaccharides demands high-resolution HPLC columns for their purification (1,2). Reverse-phase (C18) and normal-phase (NH2) columns have been used for the separation (singly or in concert) of many N-linked oligosaccharides. The porous graphitized carbon (PGC) column described in Chapter 106 for O-linked alditol separation will give improved N-linked oligosaccharide resolution over C18 columns, and has the advantage of using salt-free buffers for preparative work (3,4).

Keywords

Vast Array Control Software Reductive Amination Preparative Work Pulse Amperometric Detection 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Hase, S. (1993) Analysis of sugar chains by pyridylamination, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 69–80.CrossRefGoogle Scholar
  2. 2.
    Kakehi, K. and Honda, S. (1993) Analysis of carbohydrates in glycoproteins by high performance liquid chromatography and high performance capillary electrophoresis, in Methods in Molecular Biology, vol. 14: Glycoprotein Analysis in Biomedicine (Hounsell, E. F., ed.), Humana, Totowa, NJ, pp. 81–98.CrossRefGoogle Scholar
  3. 3.
    Smith, D. D., Davies, M. J., Hounsell, E. F. (1994) Structural profing of oligosaccharides of glycoproteins, in Methods in Molecular Biology, vol. 32: Basic Protein and Peptide Protocols (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 143–155.CrossRefGoogle Scholar
  4. 4.
    Davies, M. J. and Hounsell, E. F. (1995) Comparison of separation modes for high performance liquid chromatography of glycoprotein-and proteoglycan-derived oligosaccharides. J. Chromatogr. 720, 227–234.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Elizabeth F. Hounsell
    • 1
  • Michael J. Davies
    • 1
  • Kevin D. Smith
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUniversity College LondonUK
  2. 2.Department of Pharmaceutical SciencesUniversity of StrathclydeGlasgowScotland, UK

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